{"manuscript_title":"<b>#MATH#</b><b>CD36</b><b> </b><b>promotes</b><b> </b><b>chemotherapy</b><b> </b><b>resistance</b><b> </b><b>and</b><b> </b><b>stemness</b><b> </b><b>of cervical cancer cells through Hippo and ERK5 pathway </b><b>activation</b>","abstract":"Our previous work revealed that CD36 accelerates progression of cervical cancer. Here, we elucidate the effects of CD36 in regulating stemness and chemotherapy resistance of cervical cancer cells. We used bioinformatics analysis to assess the correlation between CD36 expression and chemotherapy drugs sensitivity in advanced cervical cancer. The effects of CD36 expression on proliferation of cervical cancer cells were analyzed using the CCK8 and colony formation assay. The stemness of cervical cancer cells were assessed by tumorsphere formation assay. Correlations between CD36 expression and stem cell markers were investigated by Western blot. The roles of CD36 on chemotherapy resistance of cervical cancer cells were evaluated by using colony formation assay, CCK8 assay and flow cytometry. Finally, the molecular mechanisms by which CD36 promotes stemness and chemotherapy resistance of cervical cancer cells were explored through bioinformatics analysis and Western blot. CD36 expression was positively correlated with cisplatin resistance in advanced cervical cancer. CD36 overexpression promoted the stemness and chemotherapy resistance of cervical cancer cells. Most importantly, we discovered that CD44, Hippo and MEK5/ERK5 pathways might be involved in CD36-mediated chemotherapy resistance and stemness of cervical cancer cells. CD36 may be an effective therapeutic target for improving the prognosis of cervical cancer.","keywords":["CD36","Stemness","Cervical cancer","Chemotherapy resistance"]}